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Malaria pp 465-479 | Cite as

Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines

  • Ian CockburnEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Abstract

The study of pathogen immunity relies upon being able to track antigen specific immune responses and assess their protective capacity. To study immunity to Plasmodium antigens, chimeric rodent or human malaria parasites that express proteins from other Plasmodium species or unrelated species have been developed. Different types of chimeric parasites have been used to address a range of specific questions. Parasites expressing model T cell epitopes have been used to monitor cellular immune responses to the preerythrocytic and blood stages of malaria. Other parasites have been used to assess the functional significance of immune responses targeting particular proteins. Finally, a number of rodent malaria parasites that express vaccine-candidate antigens from P. falciparum and P. vivax have been used in functional assays of vaccine-induced antibody responses. Here, I review the experimental contributions that have been made using these parasites, and discuss the potential of these approaches to continue advancing our understanding of malaria immunology and vaccine research.

Key words

Plasmodium Transgenic parasites Vaccines T cells Antibodies 

Notes

Acknowledgments

I thank Andrea Radtke and Fidel Zavala (Johns Hopkins University) for their critical reading of the manuscript. Work in the Zavala laboratory is supported by NIH Grant number AI44375, the Johns Hopkins Malaria Research Institute and the Bloomberg Family Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Molecular Microbiology and ImmunologyJohn Hopkins Bloomberg School of Public HealthBaltimoreUSA

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