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Malaria pp 27-33 | Cite as

In Vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes

  • Anil K. Ghosh
  • Marcelo Jacobs-LorenaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Abstract

The ookinete is the motile form of the malaria parasite that invades the mosquito midgut epithelium to initiate sporogony. Differentiation of ingested gametocytes into ookinetes in the mosquito midgut lumen and the subsequent interaction with the luminal surface of the midgut epithelium in preparation for invasion are complex processes. To facilitate the study of these events in detail, it is necessary to produce sufficient numbers of pure, fully mature ookinetes. However, production of even a small number of Plasmodium falciparum ookinetes in vitro has proven to be a daunting task. Consequently, over the past four decades our collective understanding of the biology of this parasite form remains sorely deficient. Here, we describe a new culture technique, which improves the in vitro transformation efficiency of P. falciparum gametocytes into mature ookinetes and supports the complete development of ookinetes that retain the ability to infect the mosquito midgut and to produce oocysts.

Key words

P. falciparum gametocytes Ookinetes Gametocytes to ookinete transformation 

Notes

Acknowledgments

This work was supported by grants from the National Institutes of Health, by the Johns Hopkins Malaria Research Institute and by the Bloomberg Family Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Molecular Microbiology and ImmunologyJohns Hopkins University School of Public HealthBaltimoreUSA

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