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Malaria pp 371-383 | Cite as

Scoring Sporozoite Motility

  • Allison F. Carey
  • Robert MénardEmail author
  • Daniel Y. Bargieri
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Abstract

Sporozoites, the stage of Plasmodium infectious to vertebrates when injected in the skin by a mosquito vector, are highly motile cells. Their unusual form of gliding motility is essential for infectivity, allowing the parasite to travel through both the mosquito and mammalian hosts, invading different cell types and escaping immune cell-mediated death. In this chapter, we describe techniques to study gliding motility of sporozoites in vitro and in vivo.

Key words

Malaria Sporozoites Gliding Motility Microscopy Imaging 

Notes

Acknowledgments

We thank Pauline Formaglio and Laura Schnettger for reading and discussing the manuscript.

References

  1. 1.
    Heintzelman MB (2006) Cellular and molecular mechanics of gliding locomotion in eukaryotes. Int Rev Cytol 251:79–129PubMedCrossRefGoogle Scholar
  2. 2.
    Munter S et al (2009) Plasmodium sporozoite motility is modulated by the turnover of discrete adhesion sites. Cell Host Microbe 6:551–562PubMedCrossRefGoogle Scholar
  3. 3.
    Stewart MJ, Vanderberg JP (1998) Malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility. J Protozool 35:389–393Google Scholar
  4. 4.
    Vanderberg JP (1974) Studies on the motility of Plasmodium sporozoites. J Protozool 21:527–537PubMedGoogle Scholar
  5. 5.
    Frevert U et al (2005) Intravital observation of Plasmodium berghei sporozoite infection of the liver. PLoS Biol 3:e192PubMedCrossRefGoogle Scholar
  6. 6.
    Tardieux I, Menard R (2008) Migration of Apicomplexa across biological barriers: the Toxoplasma and Plasmodium rides. Traffic 9:627–635PubMedCrossRefGoogle Scholar
  7. 7.
    Vanderberg JP, Frevert U (2004) Intravital microscopy demonstrating antibody-mediated immobilisation of Plasmodium berghei sporozoites injected into skin by mosquitoes. Int J Parasitol 34:991–996PubMedCrossRefGoogle Scholar
  8. 8.
    Matuschewski K (2006) Getting infectious: formation and maturation of Plasmodium sporozoites in the Anopheles vector. Cell Microbiol 8:1547–1556PubMedCrossRefGoogle Scholar
  9. 9.
    Amino R et al (2006) Quantitative imaging of Plasmodium transmission from mosquito to mammal. Nat Med 12:220–224PubMedCrossRefGoogle Scholar
  10. 10.
    Mota MM et al (2001) Migration of Plasmodium sporozoites through cells before infection. Science 291:141–144PubMedCrossRefGoogle Scholar
  11. 11.
    Kebaier C, Vanderberg JP (2010) Initiation of Plasmodium sporozoite motility by albumin is associated with induction of intracellular signalling. Int J Parasitol 40:25–33PubMedCrossRefGoogle Scholar
  12. 12.
    Hellmann JK et al (2011) Environmental constraints guide migration of malaria parasites during transmission. PLoS Pathog 7:e1002080PubMedCrossRefGoogle Scholar
  13. 13.
    Hegge S et al (2009) Automated classification of Plasmodium sporozoite movement patterns reveals a shift towards productive motility during salivary gland infection. Biotechnol J 4:903–913PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Allison F. Carey
    • 1
  • Robert Ménard
    • 1
    Email author
  • Daniel Y. Bargieri
    • 1
  1. 1.Unité de Biologie et Génétique du PaludismeInstitut PasteurParisFrance

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