Abstract
Chromatin immunoprecipitation (ChIP) studies have been used extensively in recent years to study the functional role of histone marks, variant histones, and other chromatin factors in gene expression in the human malaria parasite, Plasmodium falciparum. In this chapter, we present a ChIP-sequencing protocol optimized for blood-stage forms of this parasite. The processing of the immunoprecipitated DNA prior to high-throughput sequencing is performed in a way to minimize amplification biases due to the high genomic AT-content of the parasite.
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Acknowledgments
This work was supported by the French Agency for Research (ANR Blanc 0274-01) and European Research Council Executive Agency Advanced Grant (PlasmoEscape 250320). T.N.S. was supported by a Human Frontier Science Program (HFSP) fellowship.
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Lopez-Rubio, JJ., Siegel, T.N., Scherf, A. (2012). Genome-wide Chromatin Immunoprecipitation-Sequencing in Plasmodium . In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_23
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DOI: https://doi.org/10.1007/978-1-62703-026-7_23
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