Malaria pp 299-305 | Cite as

Atomic Force Microscopy of Plasmodium-Infected Red Blood Cells: Detecting and Localizing Single Molecular Recognition Events

  • Ang Li
  • Laurent Rénia
  • Chwee Teck Lim
  • Bruce RussellEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 923)


Atomic Force Microscopy (AFM) is a powerful tool for exploring the interaction between ligands and receptors, as well as their exact locations on the red cell surface. Here we discuss current and future applications for AFM based single-molecule force spectroscopy to study adhesion of Plasmodium-infected red blood cells. A protocol is provided for simultaneous topography and recognition imaging of the surface of Plasmodium falciparum-infected cells using CD36 functionalized tips.

Key words

Atomic force microscopy Single-molecule force spectroscopy Ligand–receptor ­interactions PfEMP1 CD36 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ang Li
    • 1
  • Laurent Rénia
    • 2
  • Chwee Teck Lim
    • 3
  • Bruce Russell
    • 2
    Email author
  1. 1.Singapore-MIT Alliance for Research and TechnologySingaporeSingapore
  2. 2.Singapore Immunology Network, Agency for Science, Technology and Research, BiopolisSingaporeSingapore
  3. 3.Division of Bioengineering, Department of Mechanical EngineeringNational University of SingaporeSingaporeSingapore

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