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Malaria pp 151-159 | Cite as

Transient Transfection of Plasmodium vivax Blood-Stage Parasites

  • Cecilia P. Sanchez
  • Judith Pfahler
  • Hernando A. del Portillo
  • Michael LanzerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Abstract

Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating interactions with the human host still awaits further technological breakthroughs, foremost the establishment of a continuous in vitro culture system. Nevertheless, the first steps towards domesticating P. vivax for research purposes have been made. Transfection will eventually help to better understand the unique pathogenic features displayed by P. vivax, such as the host cell specificity for reticulocytes and the occurrence of relapses. Transfection will also be an invaluable tool for studies related to the emerging drug resistance, and it will help identify and validate novel targets for rational intervention.

Key words

Transfection P. vivax Luciferase assay Saimiri monkey Ex vivo culture 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Cecilia P. Sanchez
    • 1
  • Judith Pfahler
    • 1
  • Hernando A. del Portillo
    • 2
  • Michael Lanzer
    • 1
    Email author
  1. 1.Department of Infectious DiseasesUniversitätsklinikum HeidelbergHeidelbergGermany
  2. 2.Barcelona Centre for International Health ResearchBarcelonaSpain

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