Mouse Models for Tumor Metastasis
Tumor metastasis is the main cause of death of cancer patients. Here we describe two mouse models for investigating tumor metastasis. In the first spontaneous metastasis mouse model, 4T1 mouse breast tumor cells are injected into the mammary gland of host mice and the metastasis of 4T1 tumor cells into the lung are examined with a colonogenic assay. In the second experimental metastasis mouse model, luciferase-labeled MDA-MB-231 human breast tumor cells are injected into the tail vein of NOD-SCID immunodeficient mice and the colonization of MDA-MB-231 tumor cells in the lung are monitored using noninvasive bioluminescence imaging.
Key wordsTumwor metastasis 4T1 mouse breast tumor cells MDA-MB-231 human breast tumor cells Allograft Xenograft
This work was supported by NIH grant R01CA136837 to Xin-Yun Huang and a Career Development Award from Donald A. Adam Comprehensive Melanoma Research Center to Shengyu Yang.
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