Mouse Models for Tumor Metastasis

  • Shengyu Yang
  • J. Jillian Zhang
  • Xin-Yun HuangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 928)


Tumor metastasis is the main cause of death of cancer patients. Here we describe two mouse models for investigating tumor metastasis. In the first spontaneous metastasis mouse model, 4T1 mouse breast tumor cells are injected into the mammary gland of host mice and the metastasis of 4T1 tumor cells into the lung are examined with a colonogenic assay. In the second experimental metastasis mouse model, luciferase-labeled MDA-MB-231 human breast tumor cells are injected into the tail vein of NOD-SCID immunodeficient mice and the colonization of MDA-MB-231 tumor cells in the lung are monitored using noninvasive bioluminescence imaging.

Key words

Tumwor metastasis 4T1 mouse breast tumor cells MDA-MB-231 human breast tumor cells Allograft Xenograft 



This work was supported by NIH grant R01CA136837 to Xin-Yun Huang and a Career Development Award from Donald A. Adam Comprehensive Melanoma Research Center to Shengyu Yang.


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Shengyu Yang
    • 1
    • 2
  • J. Jillian Zhang
    • 3
  • Xin-Yun Huang
    • 3
    Email author
  1. 1.Department of PhysiologyCornell University Weill Medical CollegeNew YorkUSA
  2. 2.Department of Molecular OncologyMoffitt Cancer CenterTampaUSA
  3. 3.Department of PhysiologyCornell University Weill Medical CollegeNew YorkUSA

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