Advertisement

Mouse Models for Tumor Metastasis

  • Shengyu Yang
  • J. Jillian Zhang
  • Xin-Yun HuangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 928)

Abstract

Tumor metastasis is the main cause of death of cancer patients. Here we describe two mouse models for investigating tumor metastasis. In the first spontaneous metastasis mouse model, 4T1 mouse breast tumor cells are injected into the mammary gland of host mice and the metastasis of 4T1 tumor cells into the lung are examined with a colonogenic assay. In the second experimental metastasis mouse model, luciferase-labeled MDA-MB-231 human breast tumor cells are injected into the tail vein of NOD-SCID immunodeficient mice and the colonization of MDA-MB-231 tumor cells in the lung are monitored using noninvasive bioluminescence imaging.

Key words

Tumwor metastasis 4T1 mouse breast tumor cells MDA-MB-231 human breast tumor cells Allograft Xenograft 

Notes

Acknowledgment

This work was supported by NIH grant R01CA136837 to Xin-Yun Huang and a Career Development Award from Donald A. Adam Comprehensive Melanoma Research Center to Shengyu Yang.

References

  1. 1.
    Weiss L (2000) Metastasis of cancer: a conceptual history from antiquity to the 1990s. Cancer Metastasis Rev 19:I–XI, 193–383Google Scholar
  2. 2.
    Fidler IJ (2003) The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited. Nat Rev Cancer 3:453–458PubMedCrossRefGoogle Scholar
  3. 3.
    Hanahan D, Weinberg RA (2000) The hallmarks of cancer. Cell 100:57–70PubMedCrossRefGoogle Scholar
  4. 4.
    Christofori G (2006) New signals from the invasive front. Nature 441:444–450PubMedCrossRefGoogle Scholar
  5. 5.
    Partin AW, Schoeniger JS, Mohler JL, Coffey DS (1989) Fourier analysis of cell motility: correlation of motility with metastatic potential. Proc Natl Acad Sci USA 86:1254–1258PubMedCrossRefGoogle Scholar
  6. 6.
    Aznavoorian S, Murphy AN, Stetler-Stevenson WG, Liotta LA (1993) Molecular aspects of tumor cell invasion and metastasis. Cancer 71:1368–1383PubMedCrossRefGoogle Scholar
  7. 7.
    Condeelis J, Singer RH, Segall JE (2005) The great escape: when cancer cells hijack the genes for chemotaxis and motility. Annu Rev Cell Dev Biol 21:695–718PubMedCrossRefGoogle Scholar
  8. 8.
    Shan D, Chen L, Njardarson JT, Gaul C, Ma X, Danishefsky SJ, Huang XY (2005) Synthetic analogues of migrastatin that inhibit mammary tumor metastasis in mice. Proc Natl Acad Sci USA 102:3772–3776PubMedCrossRefGoogle Scholar
  9. 9.
    Yang S, Zhang JJ, Huang XY (2009) Orai1 and STIM1 are critical for breast tumor cell migration and metastasis. Cancer Cell 15:124–134PubMedCrossRefGoogle Scholar
  10. 10.
    Chen L, Yang S, Jakoncic J, Zhang JJ, Huang XY (2010) Migrastatin analogues target fascin to block tumour metastasis. Nature 464: 1062–1066PubMedCrossRefGoogle Scholar
  11. 11.
    Mitsuhashi M, Liu J, Cao S, Shi X, Ma X (2004) Regulation of interleukin-12 gene expression and its anti-tumor activities by prostaglandin E2 derived from mammary carcinomas. J Leukoc Biol 76:322–332PubMedCrossRefGoogle Scholar
  12. 12.
    Pulaski BA, Ostrand-Rosenberg S (1998) Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res 58:1486–1493PubMedGoogle Scholar
  13. 13.
    Minn AJ, Kang Y, Serganova I, Gupta GP, Giri DD, Doubrovin M, Ponomarev V, Gerald WL, Blasberg R, Massague J (2005) Distinct organ-specific metastatic potential of individual breast cancer cells and primary tumors. J Clin Invest 115:44–55PubMedGoogle Scholar
  14. 14.
    Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB, Gerald WL, Massague J (2005) Genes that mediate breast cancer metastasis to lung. Nature 436: 518–524PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Shengyu Yang
    • 1
    • 2
  • J. Jillian Zhang
    • 3
  • Xin-Yun Huang
    • 3
    Email author
  1. 1.Department of PhysiologyCornell University Weill Medical CollegeNew YorkUSA
  2. 2.Department of Molecular OncologyMoffitt Cancer CenterTampaUSA
  3. 3.Department of PhysiologyCornell University Weill Medical CollegeNew YorkUSA

Personalised recommendations