Abstract
The redistribution and trafficking patterns of cells to different anatomic sites throughout the body is important during cancer development and metastasis. Interest in the origin and fate of gastric cancer stem cells has recently arisen, as it may explain the underlying mechanism of cancer development. The ability to monitor the migration patterns of cancer stem cells is imperative to understanding the functional changes associated with the migration and proliferation of these cells. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment of donor cells within the recipient.
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References
Houghton J, Li H, Fan X, Liu Y, Liu JH, Rao VP, Poutahidis T, Taylor CL, Jackson EA, Hewes C, Lyle S, Cerny A, Bowen G, Cerny J, Moore N, Kurt-Jones EA, Erdman SE (2010) Mutations in bone marrow-derived stromal stem cells unmask latent malignancy. Stem Cells Dev 19:115–1166
Houghton J, Nomura AB, Rogers SCS, Carlson J, Li H, Cai X, Fox JG, Goldenring JR, Wang TC (2004) Gastric cancer originating from bone marrow-derived cells. Science 306:1568–1571
Quante M, Tu SP, Tomita H, Gonda T, Wang SS, Takashi S, Baik GH, Shibata W, Diprete B, Betz KS, Friedman R, Varro A, Tycko B, Wang TC (2011) Bone marrow-derived myofibroblasts contribute to the mesenchymal stem cell niche and promote tumor growth. Cancer Cell 19:257–272
Takaishi S, Okumura T, Wang TC (2008) Gastric cancer stem cells. J Clin Oncol 26:2876–2882
Mor G, Yin G, Chefetz I, Yang Y, Alvero A (2011) Ovarian cancer stem cells and inflammation. Cancer Biol Ther 11(8):708–713
Fang D, Nguyen TK, Leishear K, Finko R, Kulp AN, Hotz S, Van Belle PA, Xu X, Elder DE, Herlyn M (2005) A tumorigenic subpopulation with stem cell properties in melanomas. Cancer Res 65:9328–9337
Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB (2004) Identification of human brain tumour initiating cells. Nature 432:396–401
Takaishi S, Okumura T, Tu S, Wang SS, Shibata W, Vigneshwaran R, Gordon SA, Shimada Y, Wang TC (2009) Identification of gastric cancer stem cells using the cell surface marker CD44. Stem Cells 27:1006–1020
Hendrikx PJ, Vermeulen J, Hagenbeek A, Vermey M, Martens AC (1996) LacZ staining in paraffin-embedded tissue sections. J Histochem Cytochem 44:1323–1329
Zeller R (2001) Fixation, embedding, and sectioning of tissues, embryos, and single cells. Curr Protoc Mol Biol, Chapter 14, Unit 14.1
Rens W, Yang F, Welch G, Revell S, O’Brien PC, Solanky N, Johnson LA, Ferguson Smith MA (2001) An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures. Reproductuion 121:541–546
Barch MJ et al (1997) The agt cytogenetics laboratory manual, 3rd edn. Lippincott-Raven, New York
Acknowledgments
The author would like to acknowledge the expert technical assistance of Kathleen LaSance (Lab Manager) and Dr. Lisa Lemen (Director) in the Vontz Core Imaging Laboratory (VCIL, University of Cincinnati). This work was supported by NIH 1R01DK083402-01A2 grant (Y. Zavros).
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Craig, M., Schumacher, M., Zavros, Y. (2012). Techniques for Following Labeled Cells In Vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-Galactosidase Detection. In: Houghton, J. (eds) Helicobacter Species. Methods in Molecular Biology, vol 921. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-005-2_25
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DOI: https://doi.org/10.1007/978-1-62703-005-2_25
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