Abstract
Pulsed-field gel electrophoresis (PFGE) is a technique for resolving large (up to 10 Mb) DNA molecules. Using multiple pairs of electrodes DNA is subject to an alternating electric field through a solid agarose matrix. As the current changes direction the reorientation time of DNA is proportional to molecular weight; thus fragments are separated in the gel based on their size. Here we describe the use of PFGE to analyze DNA double-strand break formation and repair in human chromosomal DNA.
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© 2012 Springer Science+Business Media New York
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Bryant, H.E. (2012). DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis. In: Bjergbæk, L. (eds) DNA Repair Protocols. Methods in Molecular Biology, vol 920. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-998-3_22
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DOI: https://doi.org/10.1007/978-1-61779-998-3_22
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-997-6
Online ISBN: 978-1-61779-998-3
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