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Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCR

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DNA Repair Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 920))

Abstract

The formation and repair of DNA damage at specific locations in the genome is modulated by DNA sequence context, by DNA cytosine-5 methylation patterns, by the transcriptional status of the locus and by proteins associated with the DNA. The only method currently available to allow precise sequence mapping of DNA lesions in mammalian cells is the ligation-mediated polymerase chain reaction (LM-PCR) technique. We provide an update on technical details of LM-PCR. LM-PCR can be used, for example, for mapping of ultraviolet (UV) light-induced DNA photoproducts such as cyclobutane pyrimidine dimers.

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Acknowledgement

This work was supported by a grant from the National Institute of Environmental Health Sciences (ES06070) to G.P.P.

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Authors and Affiliations

  1. Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA

    Ahmad Besaratinia & Gerd P. Pfeifer

Authors
  1. Ahmad Besaratinia
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  2. Gerd P. Pfeifer
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Corresponding author

Correspondence to Gerd P. Pfeifer .

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Editors and Affiliations

  1. , Department of Molecular Biology, Aarhus University, C.F Møllers Allé bygning 1130, Århus C, 8000, Denmark

    Lotte Bjergbæk

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Besaratinia, A., Pfeifer, G.P. (2012). Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCR. In: Bjergbæk, L. (eds) DNA Repair Protocols. Methods in Molecular Biology, vol 920. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-998-3_14

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  • DOI: https://doi.org/10.1007/978-1-61779-998-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-997-6

  • Online ISBN: 978-1-61779-998-3

  • eBook Packages: Springer Protocols

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