In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy

Part of the Methods in Molecular Biology book series (MIMB, volume 913)


Both nitric oxide (NO) and reactive oxygen species (ROS) are versatile molecules that mediate a variety of cellular responses in plants. In this chapter, methods for imaging NO and ROS using laser scanning confocal microscopy (LSCM) are presented. Arabidopsis roots, dyed with DAF-FM or H2DCF, are observed using the Leica TCS-SP2 LSCM. NO or ROS production are imaged and their kinetic changes monitored with the laser excitation and emission wavelengths at 488 nm and between 500 and 530 nm, respectively. In addition, Leica software is employed to visualize and calculate the fluorescence intensity data.

Key words

Nitric oxide Reactive oxygen species Laser scanning confocal microscopy 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.College of Life SciencesNanjing Agricultural UniversityNanjingChina

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