Abstract
Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.
Key words
- Phage display
- Intrabody
- Live cell imaging
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Acknowledgements
This work was supported by the Centre National de la Recherche Scientifique (CNRS) and the Institut Curie. FP’s project has been supported by the Agence National de la Recherche (ANR), the Institut National du Cancer (INCa), and the Agence Nationale de Recherche sur le SIDA (ANRS).
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Moutel, S., Nizak, C., Perez, F. (2012). Selection and Use of Intracellular Antibodies (Intrabodies). In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_37
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DOI: https://doi.org/10.1007/978-1-61779-974-7_37
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-974-7
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