Antibody Engineering pp 627-643 | Cite as
Recombinant Immunotoxins with Low Endotoxins for Clinical and Animal Studies
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Abstract
Recombinant immunotoxin (RIT) contains the Fv portion of the antibody fused to the truncated form of toxin and are ongoing in clinical trials for cancer therapy. To obtain high yields of products, RITs are produced in Escherichia coli (E. coli). As the endotoxin came from E. coli cells and is harmful to animals, it is important to produce the RITs with low endotoxin. This section describes the protocols to produce RITs containing low level of endotoxins.
Key words
Immunotoxin Disulfide-stabilized Fv Endotoxin Protein purification Targeting therapy ImmunotherapyNotes
Acknowledgments
The author thanks Dr. Ira Pastan, laboratory chief, and colleagues of the Laboratory of Molecular Biology for valuable discussions. This research was supported by the Intramural Research Program of the NCI, NIH, Center for Cancer Research
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