Abstract
Affinity determination is a crucial step of an antibody characterization. Here, we describe a method for antibody affinity determination by flow cytometry, relying on the unique affinity of biotin for streptavidin for easy and efficient antibody labeling. Several labeling approaches are described and discussed in this chapter, including chemical and enzymatic (in vivo and in vitro) biotinylation. Finally, a procedure for K D determination by flow cytometry is precisely described.
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References
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© 2012 Springer Science+Business Media, LLC
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Even-Desrumeaux, K., Chames, P. (2012). Affinity Determination of Biotinylated Antibodies by Flow Cytometry. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_25
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DOI: https://doi.org/10.1007/978-1-61779-974-7_25
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-973-0
Online ISBN: 978-1-61779-974-7
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