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Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

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Abstract

Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.

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Acknowledgements

This work was funded by the Science Foundation Ireland (SFI) and National University of Ireland Galway Millennium Fund 2008. HPMEC-ST1.6R cells were kindly provided by Ronald E. Unger, Institute of Pathology, Johannes Gutenberg University, Mainz, Germany.

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Authors and Affiliations

  1. National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland

    Babu R. Prasad, Enda O’Connell, Terry J. Smith & Yury Rochev

  2. CRANN and the School of Chemistry, Trinity College Dublin, Dublin, Ireland

    Valérie A. Gérard & Yurii K. Gun’ko

Authors
  1. Babu R. Prasad
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  2. Enda O’Connell
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  3. Terry J. Smith
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  4. Valérie A. Gérard
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  5. Yurii K. Gun’ko
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  6. Yury Rochev
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Corresponding author

Correspondence to Yury Rochev .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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Prasad, B.R., O’Connell, E., Smith, T.J., Gérard, V.A., Gun’ko, Y.K., Rochev, Y. (2012). Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_12

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  • DOI: https://doi.org/10.1007/978-1-61779-953-2_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

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