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The Application of Transgenic Mice for Therapeutic Antibody Discovery

  • E-Chiang LeeEmail author
  • Michael Owen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 901)

Abstract

In 2006, panitumumab, the first fully human antibody generated from transgenic mice, was approved for clinical use by the US Food and Drug Administration (FDA). Since then, a further seven such antibodies have been approved. In this chapter, we discuss how transgenic mice technologies can provide a powerful platform for creating human therapeutic antibodies.

Key words

ES cells Homologous recombination Human antibody Humanized mice Ig locus Immunoglobulin Isotype Phage display Transgenic mice Therapeutic antibody 

Notes

Acknowledgments

We thank Allan Bradley, Andrew Sandham, and Glenn Friedrich for critical comments, and all other colleagues at Kymab for helpful discussion.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Kymab Ltd, MeditrinaCambridgeUK

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