Selection of Human Fab Libraries by Phage Display

  • Christoph RaderEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 901)


This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.

Key words

Immune antibody repertoires Antibody libraries Fab Human monoclonal antibodies 



This work was supported by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, National Institutes of Health. I thank the current and former members of my laboratory, in particular Dr. Ka Yin Kwong, Dr. Jiahui Yang, and Jessica M. Suschak, for their contributions to this protocol.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Experimental Transplantation and Immunology Branch, Center for Cancer ResearchNational Cancer Institute, National Institutes of HealthBethesdaUSA
  2. 2.Department of Cancer Biology and Department of Molecular TherapeuticsThe Scripps Research InstituteScripps Florida JupiterUSA

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