Selection of Human Fab Libraries by Phage Display
This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.
Key wordsImmune antibody repertoires Antibody libraries Fab Human monoclonal antibodies
This work was supported by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, National Institutes of Health. I thank the current and former members of my laboratory, in particular Dr. Ka Yin Kwong, Dr. Jiahui Yang, and Jessica M. Suschak, for their contributions to this protocol.
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