Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity KD can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.
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Leonard P, Hayes CJ, O’Kennedy R (2011) Rapid temperature-dependent antibody ranking using Biacore A100. Anal Biochem 409: 290–292PubMedCrossRefGoogle Scholar
Roos H, Karlsson R, Nilshans H et al (1998) Thermodynamic analysis of protein interactions with biosensor technology. J Mol Recognit 11: 204–210PubMedCrossRefGoogle Scholar
Young L, Jernigan RL, Covell DG (1994) A role for surface hydrophobicity in protein–protein recognition. Protein Sci 3:717–729PubMedCrossRefGoogle Scholar
Willcox BE, Gao GF, Wyer JR et al (1999) TCR binding to peptide-MHC stabilizes a flexible recognition interface. Immunity 10:357–365PubMedCrossRefGoogle Scholar
Gabdoulline RR, Wade RC (2001) Protein-protein association: investigation of factors influencing association rates by brownian dynamics simulations. J Mol Biol 306:1139–1155PubMedCrossRefGoogle Scholar
Wang Y, Shen BJ, Sebald W (1997) A mixed-charge pair in human interleukin 4 dominates high-affinity interaction with the receptor alpha chain. Proc Natl Acad Sci U S A 94:1657–1662PubMedCrossRefGoogle Scholar
Stites WE (1997) Proteinminus signProtein interactions: interface structure, binding thermodynamics, and mutational analysis. Chem Rev 97:1233–1250PubMedCrossRefGoogle Scholar
Selzer T, Schreiber G (1999) Predicting the rate enhancement of protein complex formation from the electrostatic energy of interaction. J Mol Biol 287:409–419PubMedCrossRefGoogle Scholar
Zeder-Lutz G, Zuber E, Witz J et al (1997) Thermodynamic analysis of antigen–antibody binding using biosensor measurements at different temperatures. Anal Biochem 246: 123–132PubMedCrossRefGoogle Scholar
Pasqualucci L, Guglielmino R, Houldsworth J et al (2004) Expression of the AID protein in normal and neoplastic B cells. Blood 104: 3318–3325PubMedCrossRefGoogle Scholar
Torres M, Fernandez-Fuentes N, Fiser A et al (2007) Exchanging murine and human immunoglobulin constant chains affects the kinetics and thermodynamics of antigen binding and chimeric antibody autoreactivity. PLoS One 2:e1310PubMedCrossRefGoogle Scholar