Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

  • Michael SchrämlEmail author
  • Leopold von Proff
Part of the Methods in Molecular Biology book series (MIMB, volume 901)


Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.

Key words

Surface plasmon resonance SPR Kinetics ka kd KD Temperature Thermodynamics 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Roche Diagnostics GmbHPenzbergGermany

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