Kinetic Screening in the Antibody Development Process

  • Michael SchrämlEmail author
  • Matthias Biehl
Part of the Methods in Molecular Biology book series (MIMB, volume 901)


Kinetic screening is of paramount importance when it is to select custom-made antibodies, tailored for their respective scientific, diagnostic, or pharmaceutical application. Here a kinetic screening protocol is described, using a Biacore A100 surface plasmon resonance biosensor instrument. The assay is based on an Fc-specific antibody capture system. Antibodies from complex mixtures, like from mouse hybridoma supernatants are captured on the sensor surface in an oriented manner. The method uses a single injection of one antigen concentration for the determination of six relevant screening parameters, which comprehensively describe the antibody’s kinetic rate profile and its valence mode. The method enables the scientist to rank and finally select rare and outstanding antibodies according to their kinetic signatures.

Key words

Kinetic screening Molar Ratio Valence Dissociation half-life (t1/2 dissBinding Late Stability Late Antibody Capture Level kd Surface plasmon resonance 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Roche Diagnostics GmbHPenzbergGermany

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