Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells by Epstein–Barr Virus (EBV) transformation.
Key wordsB cells Cloning Epstein–Barr Virus Human B cell subsets Human monoclonal antibodies Immortalization Polyclonal stimulation
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