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Imaging of Vaccinia Virus Entry into HeLa Cells

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Book cover Vaccinia Virus and Poxvirology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 890))

Abstract

The recently developed technique of live cell imaging has found numerous applications, including the detection of virus movements in living cells. To monitor virus motility, viruses or cellular proteins are fused with fluorescence markers and then detected by time-lapse fluorescence microscopy. These techniques allow kinetic analyses of individual virus particles in motion during the virus entry process as well as monitoring of dynamic interactions between viruses and cellular structures in real time. The methods presented here describe how to construct a fluorescent recombinant vaccinia virus expressing the core protein A4L fused to mCherry, and how to detect the virus movement on actin-EYFP-expressed HeLa cells.

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Authors and Affiliations

  1. Academia Sinica, Institute of Molecular Biology, Taipei, Taiwan, ROC

    Cheng-Yen Huang & Wen Chang

Authors
  1. Cheng-Yen Huang
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  2. Wen Chang
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Corresponding author

Correspondence to Wen Chang .

Editor information

Editors and Affiliations

  1. School of Medicine and the Philadelphia, Division of Infectious Disease, University of Pennsylvania, Johnson Pavilion 502, Philadelphia, 19104-6073, Pennsylvania, USA

    Stuart N. Isaacs

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Huang, CY., Chang, W. (2012). Imaging of Vaccinia Virus Entry into HeLa Cells. In: Isaacs, S. (eds) Vaccinia Virus and Poxvirology. Methods in Molecular Biology, vol 890. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-876-4_7

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  • DOI: https://doi.org/10.1007/978-1-61779-876-4_7

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-875-7

  • Online ISBN: 978-1-61779-876-4

  • eBook Packages: Springer Protocols

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