Abstract
A promising in vitro zebrafish developmental toxicology assay was generated to test compounds for their teratogenic potential. The assay’s predictivity is approximately 87% in AB strain fish (Brannen KC et al., Birth Defects Res B Dev Reprod Toxicol 89:66–77, 2010). The procedure entails exposing dechorionated gastrulation-stage embryos to a range of compound concentrations for 5 days throughout embryonic and larva development. The larvae are evaluated for viability in order to identify an LC25 (the compound concentration in which 25% lethality is observed) and morphological anomalies using a numerical score system to identify the NOAEL (no observed adverse effect level). These values are used to calculate the teratogenic index (LC25/NOAEL ratio) of each compound. If the teratogenic index is equal to or greater than 10 then the compound is classified as a teratogen, and if the ratio is less than 10 then the compound is classified as a nonteratogen (Brannen KC et al., Birth Defects Res B Dev Reprod Toxicol 89:66–77, 2010).
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References
Brannen KC, Panzica-Kelly JM, Danberry TL, Augustine-Rauch KA (2010) Development of a zebrafish embryo teratogenicity assay and quantitative prediction model. Birth Defects Res B Dev Reprod Toxicol 89(1):66–77
Westerfield M (2007) The zebrafish book, 5th edn. University of Oregon Press, Eugene, OR
Panzica-Kelly JM, Zhang CX, Danberry TL, Flood A, DeLan JW, Brannen KC, Augustine-Rauch KA (2010) Morphological score assignment guidelines for the dechorionated zebrafish teratogenicity assay. Birth Defects Res B Dev Reprod Toxicol 89(5):382–395
Acknowledgments
The authors would like to thank Kimberly Brannen, Tracy Danberry, and Jeffrey Charlap for all their help in the development, optimization, and testing of this assay.
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Panzica-Kelly, J.M., Zhang, C.X., Augustine-Rauch, K. (2012). Zebrafish Embryo Developmental Toxicology Assay. In: Harris, C., Hansen, J. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 889. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-867-2_4
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DOI: https://doi.org/10.1007/978-1-61779-867-2_4
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Publisher Name: Humana Press, Totowa, NJ
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