Abstract
Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural details of individual cells and, on the other hand, are large enough to allow a correlation with light micrographs. The second protocol describes a technique for estimating mineral densities of hard tissues using backscattered electron images obtained with a scanning electron microscope. This technique can be used to analyze the mineralization processes which occur throughout tooth formation.
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Acknowledgments
The authors thank Jacqueline Hofmann-Lobsiger and Steven Reese for the skilful preparation of the specimens used for the illustrations of this work.
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Luder, H.U., Amstad-Jossi, M. (2012). Electron Microscopy. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_9
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DOI: https://doi.org/10.1007/978-1-61779-860-3_9
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