Skip to main content

Detection of Apoptosis by TUNEL Assay

  • Protocol
  • First Online:
Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 887))

Abstract

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Saraste A. (1999) Morphologic criteria and detection of apoptosis. Herz 24 (3), 189–195.

    Article  PubMed  CAS  Google Scholar 

  2. Saraste A., Pulkki K. (2000) Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 45 (3), 528–537.

    Article  PubMed  CAS  Google Scholar 

  3. Cohen J. J. (1993) Apoptosis. Immunol Today 14 (3), 126–130.

    Article  PubMed  CAS  Google Scholar 

  4. Bortner C. D., Oldenburg N. B., Cidlowski J. A. (1995) The role of DNA fragmentation in apoptosis. Trends Cell Biol 5 (1), 21–26.

    Article  PubMed  CAS  Google Scholar 

  5. Gavrieli Y., Sherman Y., Ben-Sasson S. A. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119 (3), 493–501.

    Article  PubMed  CAS  Google Scholar 

  6. Nagoescu A., Lorimier P., Labat-Moleur F., Drouet C., Robert C., Guillermet C., Brambilla C., Brambilla E. (1996) In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations. J Histochem Cytochem 44 (9), 959–968.

    Article  Google Scholar 

  7. Rode H. D., Eisel D., Frost I. (2004) Apoptosis, cell death and cell proliferation. 3rd ed. London: Roche Applied Science.

    Google Scholar 

  8. Grasl-Kraupp B., Ruttkay-Nedecky B., Koudelka H. Bukowska K., Bursch W., Schulte-Hermann R. (1995) In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note. Hepatology 21 (5), 1465–1468.

    PubMed  CAS  Google Scholar 

  9. Orita Y., Nishizaki K., Sasaki J., Kanda S., Kimura N., Nomiya S., Yuen K., Masuda Y. (1999) Does TUNEL staining during peri- and post-natal development of the mouse inner ear indicate apoptosis? Acta Otolaryngol Suppl 540, 22–26.

    PubMed  CAS  Google Scholar 

  10. Tornusciolo D. R., Schmidt R. E., Roth K. A. (1995) Simultaneous detection of TDT-mediated dUTP-biotin nick end-labeling (TUNEL)-positive cells and multiple immunohistochemical markers in single tissue ­sections. Biotechniques 19 (5), 800–805.

    PubMed  CAS  Google Scholar 

  11. Oberhaus S. M. (2003) TUNEL and immunofluorescence double-labeling assay for apoptotic cells with specific antigen(s). Methods Mol Biol 218, 85–96.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chrissa Kioussi .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Kyrylkova, K., Kyryachenko, S., Leid, M., Kioussi, C. (2012). Detection of Apoptosis by TUNEL Assay. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-860-3_5

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-859-7

  • Online ISBN: 978-1-61779-860-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics