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Detection of Apoptosis by TUNEL Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 887))


Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

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Correspondence to Chrissa Kioussi .

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© 2012 Springer Science+Business Media, LLC

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Kyrylkova, K., Kyryachenko, S., Leid, M., Kioussi, C. (2012). Detection of Apoptosis by TUNEL Assay. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press.

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-859-7

  • Online ISBN: 978-1-61779-860-3

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