Abstract
Renal gene therapy may offer new strategies to treat diseases of native and transplanted kidneys. Several experimental techniques have been developed using viral, nonviral, and cellular vectors, although the effectiveness of such techniques varies widely depending upon the vector used, type of injection, species, and experimental model of renal disease. Here, we describe an optimized technique for renal delivery of DNA in rodents by retrograde renal vein injection as it is currently applied in our laboratory for adenovirus and nonviral vectors. This is an effective gene transfer method with lasting effect on gene expression in the kidney that modulates renal disease in rodents without any apparent harmful effect, thus having a potential therapeutic value for future clinical applications.
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References
Isaka Y (2006) Gene therapy targeting kidney diseases: routes and vehicles. Clin Exp Nephrol 10:229–235
Appledorn DM, Seregin S, Amalfitano A (2008) Adenovirus vectors for renal-targeted gene delivery. Contrib Nephrol 159:47–62
Lien YH, Lai LW (2003) Renal gene transfer: nonviral approaches. Mol Biotechnol 24:283–294
Akinc A, Thomas M, Klibanov AM, Langer R (2005) Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J Gene Med 7:657–663
Bonnet ME, Erbacher P, Bolcato-Bellemin AL (2008) Systemic delivery of DNA or siRNA mediated by linear polyethylenimine (L-PEI) does not induce an inflammatory response. Pharm Res 25:2972–2982
Zou SM, Erbacher P, Remy JS, Behr JP (2000) Systemic linear polyethylenimine (L-PEI)-mediated gene delivery in the mouse. J Gene Med 2:128–134
Boletta A, Benigni A, Lutz J et al (1997) Nonviral gene delivery to the rat kidney with polyethylenimine. Hum Gene Ther 8:1243–1251
Ferrari S, Moro E, Pettenazzo A et al (1997) ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Ther 4:1100–1106
Boussif O, Lezoualc’h F, Zanta MA et al (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci USA 92:7297–7301
Wang S, Ma N, Gao SJ, Yu H, Leong KW (2001) Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes. Mol Ther 3:658–664
van der Wouden EA, Sandovici M, Henning RH, de Zeeuw D, Deelman LE (2004) Approaches and methods in gene therapy for kidney disease. J Pharmacol Toxicol Methods 50:13–24
Fujishiro J, Takeda S, Takeno Y et al (2005) Gene transfer to the rat kidney in vivo and ex vivo using an adenovirus vector: factors influencing transgene expression. Nephrol Dial Transplant 20:1385–1391
Bledsoe G, Shen B, Yao Y et al (2006) Reversal of renal fibrosis, inflammation, and glomerular hypertrophy by kallikrein gene delivery. Hum Gene Ther 17:545–555
Gong N, Dong C, Chen Z et al (2006) Adenovirus-mediated antisense-ERK2 gene therapy attenuates chronic allograft nephropathy. Transplant Proc 38:3228–3230
Sandovici M, Henning RH, van Goor H et al (2008) Systemic gene therapy with interleukin-13 attenuates renal ischemia-reperfusion injury. Kidney Int 73:1364–1373
Zhang Z, Wu F, Zheng F, Li H (2010) Adenovirus-mediated decorin gene transfection has therapeutic effects in a streptozocin-induced diabetic rat model. Nephron Exp Nephrol 116:e11–e21
Ortiz-Muñoz G, Lopez-Parra V, Lopez-Franco O et al (2010) Suppressors of cytokine signaling abrogate diabetic nephropathy. J Am Soc Nephrol 21:763–772
Hernández-Vargas P, López-Franco O, Sanjuán G et al (2005) Suppressors of cytokine signaling regulate angiotensin II-activated Janus kinase-signal transducers and activators of transcription pathway in renal cells. J Am Soc Nephrol 16:1673–1683
Ortiz-Muñoz G, Martin-Ventura JL, Hernandez-Vargas P et al (2009) Suppressors of cytokine signaling modulate JAK/STAT-mediated cell responses during atherosclerosis. Arterioscler Thromb Vasc Biol 29:525–531
Acknowledgments
The authors have been granted by Spanish Ministry of Science (SAF2005/05857, SAF2007/63648, and SAF2009/11794), Ministry of Health (Instituto de Salud Carlos III, Red RECAVA RD06/0014/0035) and Comunidad de Madrid (S2006/GEN-0247).
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Ortiz-Muñoz, G., Mallavia, B., Lopez-Franco, O., Hernandez-Vargas, P., Egido, J., Gomez-Guerrero, C. (2012). Renal Delivery of Adenovirus and Antisense Oligonucleotides in Rats by Retrograde Renal Vein Injection. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_29
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DOI: https://doi.org/10.1007/978-1-61779-851-1_29
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-851-1
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