Abstract
Design-based stereology is considered the gold-standard method for estimating the total number of glomeruli, and thereby nephrons, in the adult kidney. However, until recently, a design-based method for estimating nephron number in the developing kidney was not available. For such a method to provide accurate and precise estimates, unambiguous identification of developing nephrons is essential. Here, we describe a combined histochemical/stereological technique for estimating total nephron number in the developing mouse and rat kidney. The method can be modified for use in other species.
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© 2012 Springer Science+Business Media, LLC
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Cullen-McEwen, L.A., Armitage, J.A., Nyengaard, J.R., Bertram, J.F. (2012). Estimating Nephron Number in the Developing Kidney Using the Physical Disector/Fractionator Combination. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_10
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DOI: https://doi.org/10.1007/978-1-61779-851-1_10
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-850-4
Online ISBN: 978-1-61779-851-1
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