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Dissecting and Culturing and Imaging the Mouse Urogenital System

Protocol
First Online:
Part of the Methods in Molecular Biology™ book series (MIMB, volume 886)

Abstract

Current knowledge of the morphological and molecular events driving branching morphogenesis of the ureteric bud (UB) during development of the metanephric kidney has been greatly facilitated by the ability to explant this organ to culture. The UB can be further isolated from the mesenchyme and grown within a three-dimensional, collagen-based matrix when supplemented with the appropriate growth factors. The protocol presented here outlines the dissection and culture techniques necessary to dissect and culture the whole kidney and the isolated UB.

Key words

Kidney Ureteric bud Branching morphogenesis Organ culture Growth factor 
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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Genetics and DevelopmentColumbia UniversityNew YorkUSA
  2. 2.Welcome Trust Sanger InstituteHinxtonUK

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