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Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity

  • Michael D. Varney
  • Alison S. L. Castley
  • Katri Haimila
  • Päivi Saavalainen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 882)

Abstract

This chapter describes the application of diagnostic HLA typing for disease association and five methods used for specific HLA genotypes. The methods utilise a combination of polymerase chain reaction (PCR) amplification to detect sequence polymorphism by the presence or absence of amplification, nucleotide sequencing of the PCR product, and hybridisation of the PCR product with labelled probes. The probes are specific for sequence polymorphism associated with the genotype and are attached to either a Micro Bead or a Solid Phase. In addition, the detection of single nucleotide polymorphism(s) which “tag” for the genotype using a real-time PCR is described.

Key words

HLA Disease Association Hypersensitivity Methods B*27 B*57 Coeliac B*1502 Narcolepsy DQ 

Notes

Acknowledgements

The DR/DQ SSP, SBT, and micro bead hybridisation methods are a culmination of input by the molecular typing staff of the Transplantation and Immunogenetics Service of the Australian Red Cross Blood Service.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Michael D. Varney
    • 1
  • Alison S. L. Castley
    • 2
  • Katri Haimila
    • 3
  • Päivi Saavalainen
    • 4
  1. 1.Victorian Transplantation and Immunogenetics Service, Australian Red Cross Blood ServiceMelbourneAustralia
  2. 2.Department of Clinical Immunology, PathWest Laboratory MedicineRoyal Perth HospitalPerthAustralia
  3. 3.Clinical LaboratoryFinnish Red Cross Blood ServiceHelsinkiFinland
  4. 4.Research Program for Molecular Medicine, Department of Medical Genetics, Haartman Institute, Biomedicum HelsinkiUniversity of HelsinkiHelsinkiFinland

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