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Immunogenetics pp 351-357 | Cite as

Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node

  • Gabriella Tassone
  • Samantha J. Fidler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 882)

Abstract

Spleen and lymph node retrieved post-mortem from deceased organ donors are a rich source of lymphocytes. Storage of lymphocytes separated from these sources can be valuable where post-transplant testing (crossmatching) is required. DNA extraction from stored lymphocytes also allows further genetic testing where required, for example additional HLA typing not performed at the time of transplant for donor-specific antibody monitoring. Methods for the isolation and freezing of such cells is described.

Key words

Lymphocytes Spleen Lymph node Cryopreservation 

References

  1. 1.
    Bøyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest Suppl 97:77–87PubMedGoogle Scholar
  2. 2.
    Strong DM (2000) Cryopreservation of lymphocytes in bulk. ASHI Laboratory Manual 4th Edition, vol 1Google Scholar
  3. 3.
    Lorentzen D (2000) Cell preservation. ASHI Laboratory Manual 4th Edition, vol 1Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Clinical Immunology, PathWest Laboratory MedicineRoyal Perth HospitalPerthAustralia
  2. 2.School of Pathology and Laboratory Medicine (PaLM)University of Western AustraliaNedlandsAustralia

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