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Protein Electrophoresis pp 49–53Cite as

Native Polyacrylamide Gels

Part of the Methods in Molecular Biology book series (MIMB,volume 869)

Abstract

Usually proteins are separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed and to provide all proteins with a negative charge. As a consequence, the proteins will be separated according to their molecular weight. Electrophoresis of proteins can also be performed in the absence of SDS. Using such “native” conditions, the charge of each of the proteins, which will depend on the primary amino acid sequence of the protein (isoelectric point) and the pH during electrophoresis, will mainly influence the mobility of the respective protein during electrophoresis. Here we describe a starting protocol for “native” PAGE.

Key words

  • “Native” polyacrylamide gels
  • “Blue native” polyacrylamide gels
  • Proteins

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  • DOI: 10.1007/978-1-61779-821-4_5
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Acknowledgments

Special thanks for excellent technical support to Christin Gräfe, Livia Schulze, und Barbara Uteß (Inst. Immunology, Dresden, Germany).

Author information

Authors and Affiliations

  1. Carl Gustav Carus University TU Dresden, Dresden, Germany

    Claudia Arndt, Stefanie Koristka, Holger Bartsch & Michael Bachmann

Authors
  1. Claudia Arndt
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  2. Stefanie Koristka
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  3. Holger Bartsch
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  4. Michael Bachmann
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Corresponding author

Correspondence to Michael Bachmann .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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Arndt, C., Koristka, S., Bartsch, H., Bachmann, M. (2012). Native Polyacrylamide Gels. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_5

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols