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Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 877)

Abstract

The ability of plants to remove pollutants from the environment is currently used in a simple and low-cost cleaning technology known as phytoremediation. Unfortunately, little is known about the metabolic pathways involved in the transformation of xenobiotic compounds and the ability of certain plants to tolerate, detoxify, and store high concentrations of heavy metals. Plant cell and tissue culture is considered an important tool for fundamental studies that provide information about the plant-contaminant relationships, help to predict plant responses to environmental contaminants, and improve the design of plants with enhanced characteristics for phytoremediation. Callus, cell suspensions, hairy roots, and shoot multiplication cultures are used to study the interactions between plants and pollutants under aseptic conditions. Many plant species have an inherent ability to accumulate/metabolize a variety of pollutants, but they normally produce little biomass. However, fast-growing trees are excellent candidates for phytoremediation because of their rapid growth, extensive root system, and high water uptake. This chapter outlines the in vitro plant production of both somaclonal variants and transgenic plants of Populus spp. that exhibit high tolerance to heavy metals.

Key words

Environmental contaminants Heavy metals Micropropagation Phytochelatin synthase Phytoremediation Populus spp. Somaclonal variation Transgenic plants 
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References

  1. 1.
    Peuke AD, Rennenberg H (2005) Phytoremediation. EMBO Rep 6:497–501PubMedCrossRefGoogle Scholar
  2. 2.
    Krämer U (2005) Phytoremediation: novel approaches to clearing up polluted soils. Curr Opin Biotechnol 16:133–141PubMedCrossRefGoogle Scholar
  3. 3.
    Van Nevel L et al (2007) Phytoextraction of metals from soils: how far from practice? Environ Pollut 150:34–40PubMedCrossRefGoogle Scholar
  4. 4.
    Robinson BH et al (2006) Phytoremediation for the management of metal flux in contaminated sites. Forest Snow Landsc Res 80:221–234Google Scholar
  5. 5.
    Do Nascimento CWA, Xing B (2006) Phytoextraction: a review on enhanced metal availability and plant accumulation. Sci Agric 63:299–311CrossRefGoogle Scholar
  6. 6.
    Clemens S (2001) Molecular mechanisms of plant metal tolerance and homeostasis. Planta 212:475–486PubMedCrossRefGoogle Scholar
  7. 7.
    Clemens S et al (2002) A long way ahead: understanding and engineering plant metal accumulation. Trends Plant Sci 7:309–315PubMedCrossRefGoogle Scholar
  8. 8.
    Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832PubMedCrossRefGoogle Scholar
  9. 9.
    Tennstedt P et al (2009) Phytochelatin synthesis is essential for the detoxification of excess zinc and contributes significantly to the accumulation of zinc. Plant Physiol 149:938–948PubMedCrossRefGoogle Scholar
  10. 10.
    Sing OV, Jain RK (2003) Phytoremediation of toxic aromatic pollutants from soil. Appl Microbiol Biotechnol 63:128–135CrossRefGoogle Scholar
  11. 11.
    Krämer U, Chardonnens AN (2001) The use of transgenic plants in the bioremediation of soils contaminated with trace elements. Appl Microbiol Biotechnol 55:661–672PubMedCrossRefGoogle Scholar
  12. 12.
    Doran P (2009) Application of plant tissue cultures in phytoremediation research: incentives and limitations. Biotechnol Bioeng 103:60–76PubMedCrossRefGoogle Scholar
  13. 13.
    Peuke AD, Rennenberg H (2005) Phytore­mediation with transgenic trees. Z Naturforsch 60c:199–207Google Scholar
  14. 14.
    Merkle SA (2006) Engineering forest trees with heavy metal resistance genes. Silvae Genet 55:263–268Google Scholar
  15. 15.
    Eapen S et al (2007) Advances in development of transgenic plants for remediation of xenobiotic pollutants. Biotechnol Adv 25:442–451PubMedCrossRefGoogle Scholar
  16. 16.
    Doty SL (2008) Enhancing phytoremediation through the use of transgenics and endophytes. New Phytol 179:318–333PubMedCrossRefGoogle Scholar
  17. 17.
    Van Aken B (2008) Transgenic plants for phytoremediation: helping nature to clean up environmental pollution. Trends Biotechnol 26:225–227PubMedCrossRefGoogle Scholar
  18. 18.
    Sederoff R (2007) Regulatory science in forest biotechnology. Tree Genet Genom 3:71–74CrossRefGoogle Scholar
  19. 19.
    Strauss SH et al (2009) Strangle at birth? Forest biotech and the convention on biology diversity. Nat Biotechnol 27:519–527PubMedCrossRefGoogle Scholar
  20. 20.
    Nehnevajova E et al (2007) In vitro breeding of Brassica juncea L. to enhance metal accumulation and extraction properties. Plant Cell Rep 26:429–437PubMedCrossRefGoogle Scholar
  21. 21.
    Bittsánszky A et al (2008) In vitro breeding of grey poplar (Populus x canescens) for phytoremediation purposes. J Chem Technol Biotechnol 84:890–894CrossRefGoogle Scholar
  22. 22.
    Confalonieri M et al (2003) In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue Organ Cult 72:109–138CrossRefGoogle Scholar
  23. 23.
    Doty SL et al (2007) Enhanced phytoremediation of volatile environmental pollutants with transgenic trees. Proc Natl Acad Sci USA 104:16816–16821PubMedCrossRefGoogle Scholar
  24. 24.
    Van Dillewijn P et al (2008) Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen. Environ Sci Technol 42:7405–7410PubMedCrossRefGoogle Scholar
  25. 25.
    Couselo JL et al (2010) Expression of the phytochelatin synthase TaPCS1 in transgenic aspen, insight into the problems and qualities in phytoremediation of Pb. Int J Phytol 12:358–370CrossRefGoogle Scholar
  26. 26.
    Fry J et al (1997) Somaclonal variation in Populus: an evaluation. In: Klopfenstein NB et al (eds) Micropropagation, genetic engineering, and molecular biology of Populus. Gen Tech rep RM-GTR-297. US Department of Agriculture, Forest Service, Rocky Mountains Research Station, Fort Collins, COGoogle Scholar
  27. 27.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  28. 28.
    Hoagland DR, Arnon DI (1941) The water culture method for growing plants without soil. Miscellaneous publications nº 3514. Circular of the California Agricultural Experimental StationGoogle Scholar
  29. 29.
    Hood EE et al (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res 2:208–218CrossRefGoogle Scholar
  30. 30.
    Couselo JL, Corredoira E (2004) Trans­formación genética de Populus tremula x tremuloides con la secuencia AtPCS1 para su uso en programas de fitorremediación. Rev Real Acad Gal Cien 23:79–94Google Scholar
  31. 31.
    Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  32. 32.
    Ostry ME, Ward KT (2003) Field performance of Populus expressing somaclonal variation in resistance to Septoria musiva. Plant Sci 164:1–8CrossRefGoogle Scholar
  33. 33.
    Di Lonardo S et al (2011) Exploring the metal phytoremediation potential of three Populus alba L. clones using an in vitro screening. Environ Sci Pollut Res Int 18:82–90PubMedCrossRefGoogle Scholar
  34. 34.
    Tzfira T et al (1997) Transgenic Populus tremula: a step-by-step protocol for its Agrobacterium-mediated transformation. Plant Mol Biol Rep 15:219–235CrossRefGoogle Scholar
  35. 35.
    Gisbert C et al (2003) A plant genetically modified that accumulates Pb is especially promising for phytoremediation. Biochem Biophys Res Commun 303:440–445PubMedCrossRefGoogle Scholar
  36. 36.
    Gisbert C et al (2006) Tolerance and accumulation of heavy metals by Brassicaceae species grown in contaminated soils from Mediterranean regions of Spain. Environ Exp Bot 25:19–27CrossRefGoogle Scholar
  37. 37.
    EPA (1995) Method 1638: determination of trace elements in ambient waters by inductively coupled plasma-mass spectrometry. EPA 821-R-95-031. US Environmental Protection Agency, Office of Water, Washington, DCGoogle Scholar
  38. 38.
    Parker DR et al (1995) Geochem-PC a chemical speciation program for IBM and compatible personal computers. In: Loeppert RH, Schwab AP, Gobler S (eds) SSA special publication number 38. American Society of Agronomy, Soil Science Society of America, Madison, WIGoogle Scholar
  39. 39.
    Gyulai G et al (2005) AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro. Z Naturforsch 60:300–306Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Estación Fitopatológica do AreeiroPontevedraSpain
  2. 2.Instituto de Investigaciones Agrobiológicas de Galicia, C.S.I.C.Santiago de CompostelaSpain

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