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Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 879)

Abstract

Collection and banking of umbilical cord blood can provide a virtually unlimited source of ethnically diverse stem cell donors. It can be used in place of bone marrow or peripheral blood stem cells for hematologic transplants as well as in a variety of regenerative medicine applications. In this study, we review the latest developments in cord blood banking. We have banked over 300,000 collections at our facility, which were processed by either Ficoll or AXP methodologies. An average 95–99% processing efficiency was obtained. Processed samples can be frozen in either cryovials or bags and banked in the vapor phase of a liquid nitrogen dewar for prolonged periods of time. In conclusion, it is possible to simply and reproducibly harvest, process, and bank cord blood samples using currently available technology.

Key words

Cord blood Banking Processing Collection Stem cells 

Abbreviations

AXP

AutoXpress™ Platform

BMT

Bone marrow transplantation

CB

Cord blood

CBR

Cord Blood Registry

DMSO

Dimethylsulfoxide

GVHD

Graft-versus-host disease

HLA

Human leukocyte antigen

IDM

Infectious disease marker

MNC

Mononuclear cells

TNC

Total nucleated cells

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Notes

Acknowledgments

I would like to acknowledge the invaluable technical assistance all of the Cord Blood Bank personnel that have made this study possible. I would also like to acknowledge the numerous physicians, midwifes, and nurses that have participated in the collection of the cord blood samples.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of ImmunobiologyUniversity of ArizonaTucsonUSA

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