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Spectroscopic Methods of Analysis pp 1–22Cite as

Fluorescence Lifetime Imaging Comes of Age How to Do It and How to Interpret It

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 875))

Abstract

Fluorescence lifetime imaging (FLI) has been used widely for measuring biomedical samples. Practical guidelines on taking successful FLI data are provided to avoid common errors that arise during the measurement. Several methods for analyzing and interpreting FLI results are also introduced; e.g., a model-free data analysis method called the polar plot allows visualization and analysis of FLI data without iterative fitting, and an image denoising algorithm called variance-stabilizing-transform TI Haar helps to elucidate the information of a complex biomedical sample. The instrument considerations and data analysis of Spectral-FLI are also discussed.

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Acknowledgement

Yi-Chun Chen thanks the Taiwan Merit Scholarships (TMS-094-1-A-036) for financial support.

Author information

Authors and Affiliations

  1. Bioengineering Department, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Yi-Chun Chen & Robert M. Clegg

  2. Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Bryan Q. Spring & Robert M. Clegg

  3. Loomis Laboratory of Physics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Robert M. Clegg

Authors
  1. Yi-Chun Chen
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  2. Bryan Q. Spring
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  3. Robert M. Clegg
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Corresponding author

Correspondence to Robert M. Clegg .

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Editors and Affiliations

  1. Dept. Biochemistry & Molecular Biology, University of Texas Medical Branch, University Blvd. 301, Galveston, 77555-1053, Texas, USA

    Wlodek M. Bujalowski

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Chen, YC., Spring, B.Q., Clegg, R.M. (2012). Fluorescence Lifetime Imaging Comes of Age How to Do It and How to Interpret It. In: Bujalowski, W. (eds) Spectroscopic Methods of Analysis. Methods in Molecular Biology, vol 875. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-806-1_1

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  • DOI: https://doi.org/10.1007/978-1-61779-806-1_1

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