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A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule

  • Jun-ichi Morishige
  • Tamotsu TanakaEmail author
  • Kiyoshi Satouchi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 874)

Abstract

Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate using a ­phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples.

Key words

Sphingosine-1-phosphate Phos-tag Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Silica gel Cleanup technology 

Notes

Acknowledgment

This work was supported by the Strategic Support Project of Research Infrastructure Formation for Private Universities from the MEXT, Japan.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jun-ichi Morishige
    • 1
  • Tamotsu Tanaka
    • 2
    • 3
    Email author
  • Kiyoshi Satouchi
    • 4
  1. 1.Research Center for Green ScienceFukuyama UniversityFukuyamaJapan
  2. 2.Department of Applied Biological ScienceFukuyama UniversityFukuyamaJapan
  3. 3.Institute of Health BiosciencesUniversity of Tokushima Graduate SchoolTokushimaJapan
  4. 4.Department of Nutrition and Life ScienceFukuyama UniversityFukuyamaJapan

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