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Sphingosine-1-Phosphate Signaling in Neural Progenitors

  • Phillip Callihan
  • Shelley B. HooksEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 874)

Abstract

Sphingosine-1-phosphate (S1P) and its receptors are important in nervous system development. Reliable in vitro human model systems are needed to further define specific roles for S1P signaling in neural development. We have recently reported that human embryonic stem cell-derived neuroepithelial progenitor cells (hES-NEP) express functional S1P receptors. These cells can be further differentiated to a neuronal cell type, and therefore represent a good model system to study the role of S1P signaling in human neural development. The following sections describe in detail the culture of hES-NEP cells and two assays to measure S1P signaling in these cells.

Key words

Neural progenitor cells Stem cells Sphingosine-1-phosphate S1P cAMP Inositol phosphates Second messenger S1P receptor G-protein coupled receptor Phospholipase C Adenylyl cyclase 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Pharmaceutical and Biomedical SciencesUniversity of GeorgiaAthensUSA

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