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Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells

  • Elisabetta MeacciEmail author
  • Francesca Bini
  • Chiara Battistini
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 874)

Abstract

Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid regulator of numerous important physiological and pathological processes in mammalian and nonmammalian cells. There are emerging evidence that many cell types can produce and release S1P; therefore, the quantification of its intracellular and extracellular content as well as the activity of sphingosine kinase (SphK), the enzyme responsible of S1P synthesis, is crucial to attribute to the SphK/S1P axis a functional significance in response to many different stimuli and in physiopathological conditions.

This chapter describes experimental procedures to measure intracellular S1P formation in skeletal muscle cells and skeletal muscle fibers by using sphingolipid precursors. It also underlines the relevance of measuring S1P production in specific cellular compartments in order to attribute to S1P signaling a role in the biology of skeletal muscle cells.

Key words

Sphingosine 1-phosphate Sphingosine Thin-layer chromatography Skeletal muscle Satellite cells Lipid rafts Lipid extraction 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Elisabetta Meacci
    • 1
    • 2
    Email author
  • Francesca Bini
    • 1
  • Chiara Battistini
    • 1
  1. 1.Department of Biochemical SciencesUniversity of FlorenceFlorenceItaly
  2. 2.Interuniversity Institute of Myology (IIM)FlorenceItaly

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