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Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate

  • Leyre Brizuela
  • Olivier CuvillierEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 874)

Abstract

Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules (3H, 32P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.

Key words

Sphingolipid Ceramide Sphingosine Sphingosine-1-phosphate Sphingosine kinase-1 Diacylglycerol kinase 

Notes

Acknowledgments

This work was supported by fundings from the Institut National du Cancer (INCa), La Ligue Nationale Contre le Cancer (Equipe labellisée), La Ligue Contre le Cancer (Comité 31), Association pour la Recherche sur le Cancer (ARC), Association pour la Recherche sur les Tumeurs de la Prostate (ARTP), Université Paul Sabatier de Toulouse.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institut de Pharmacologie et de Biologie Structurale, CNRSToulouseFrance
  2. 2.UPS, IPBS, Université de ToulouseToulouseFrance

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