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Animal Models of Acute Neurological Injuries II pp 61–66Cite as

Biochemical and Molecular Biological Assessments of Global Cerebral Ischemia: Protein

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Abstract

The evaluation of protein expression and modifications following global cerebral ischemia has led to better characterization of selectively vulnerable neuronal populations and exploration of potential targets for neuroprotection. The absence or decrease of protein product must also be validated for knockdown or knockout models in order to ensure efficacy of gene silencing. Thus, two basic approaches for protein assessments (western blotting and immunohistochemistry) used in global cerebral ischemic models are presented here to serve as the basis of further experimental designs.

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References

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Author information

Authors and Affiliations

  1. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Yu Gan

  2. Geriatric Research, Educational and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA, USA

    R. Anne Stetler

  3. Center of Cerebrovascular Disease Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    R. Anne Stetler

  4. State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China

    R. Anne Stetler

Authors
  1. Yu Gan
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  2. R. Anne Stetler
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Corresponding author

Correspondence to Yu Gan .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Gan, Y., Stetler, R.A. (2012). Biochemical and Molecular Biological Assessments of Global Cerebral Ischemia: Protein. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_6

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  • DOI: https://doi.org/10.1007/978-1-61779-782-8_6

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

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