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The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (Review)

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Recombinant Protein Production in Yeast

Part of the book series: Methods in Molecular Biology ((MIMB,volume 866))

Abstract

Biological processes are subject to the influence of numerous factors and their interactions, which may be non-linear in nature. In a recombinant protein production experiment, understanding the relative importance of these factors, and their influence on the yield and quality of the recombinant protein being produced, is an essential part of its optimisation. In many cases, implementing a design of experiments (DoE) approach has delivered this understanding. This chapter aims to provide the reader with useful pointers in applying a DoE strategy to improve the yields of recombinant yeast cultures.

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Author information

Authors and Affiliations

  1. School of Life & Health Sciences and Aston Research Centre for Healthy Ageing, Aston University, Birmingham, UK

    Nagamani Bora, Zharain Bawa & Roslyn M. Bill

  2. Smallpeice Enterprises Ltd, Warwickshire, UK

    Martin D. B. Wilks

Authors
  1. Nagamani Bora
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  2. Zharain Bawa
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  3. Roslyn M. Bill
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  4. Martin D. B. Wilks
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Corresponding author

Correspondence to Martin D. B. Wilks .

Editor information

Editors and Affiliations

  1. School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, United Kingdom

    Roslyn M. Bill

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© 2012 Springer Science+business Media, LLC

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Bora, N., Bawa, Z., Bill, R.M., Wilks, M.D.B. (2012). The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (Review). In: Bill, R. (eds) Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 866. Humana Press. https://doi.org/10.1007/978-1-61779-770-5_11

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  • DOI: https://doi.org/10.1007/978-1-61779-770-5_11

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-769-9

  • Online ISBN: 978-1-61779-770-5

  • eBook Packages: Springer Protocols

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