ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism

  • Maryam SarwatEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 862)


With the emergence of more and more molecular markers as useful tools in plethora of population genetic and phylogenetic studies, choice of marker system for a particular study has become mind boggling. These marker systems differ in their advantages and disadvantages, so it is imperative to keep in mind all the pros and cons of the technique while selecting one for the problem to be addressed.

Here, we have shed light on the ISSR (intersimple sequence repeat) technique, as a marker of choice if one wants to go for properties such as reliability, simplicity, cost effectiveness, and speed, in addition to assessing genetic diversity between closely related individuals. We have outlined here the whole methodology of this technique with an example of Tribulus terrestris as case study.

Key words

DNA markers ISSR AFLP Tribulus terrestris DNA fingerprinting 



Amplified fragment length polymorphism


Arbitrarily primed PCR


Bulk segregant analysis


Cleaved amplified polymorphic sequences


DNA amplification fingerprinting


Inter-retrotransposon amplified polymorphism


Intersimple sequence repeats


Random amplification of polymorphic DNA


Retro-transposon-microsatellite amplified polymorphism


Restriction fragment length polymorphism


Selectively amplified microsatellite polymorphic loci


Sequence characterized amplified region


Sequence-related amplified polymorphism


Sequence-specific amplification polymorphism



The research work on T. terrestris is funded by a Department of Biotechnology, Government of India grant.


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© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Pharmaceutical Biotechnology, Amity Institute of PharmacyAmity UniversityNoidaIndia

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