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DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants

  • Nikolaus J. SucherEmail author
  • James R. Hennell
  • Maria C. Carles
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 862)

Abstract

DNA fingerprinting of plants has become an invaluable tool in forensic, scientific, and industrial laboratories all over the world. PCR has become part of virtually every variation of the plethora of approaches used for DNA fingerprinting today. DNA sequencing is increasingly used either in combination with or as a replacement for traditional DNA fingerprinting techniques. A prime example is the use of short, standardized regions of the genome as taxon barcodes for biological identification of plants. Rapid advances in “next generation sequencing” (NGS) technology are driving down the cost of sequencing and bringing large-scale sequencing projects into the reach of individual investigators. We present an overview of recent publications that demonstrate the use of “NGS” technology for DNA fingerprinting and DNA barcoding applications.

Key words

DNA fingerprinting DNA sequencing DNA barcoding Next generation sequencing Plant transcriptomics Chloroplast genome 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Nikolaus J. Sucher
    • 1
    Email author
  • James R. Hennell
    • 2
  • Maria C. Carles
    • 3
  1. 1.Department of Science, Technology, Engineering & Math (S.T.E.M.)Roxbury Community CollegeBostonUSA
  2. 2.Centre for Complementary Medicine Research, School of Science and HealthUniversity of Western SydneyPenrithAustralia
  3. 3.Department of Natural SciencesNorthern Essex Community CollegeHaverhillUSA

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