Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons
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Among the PCR-based markers that are most widely used in molecular genetic studies, SCARs (sequence characterized amplified regions) have the advantage of being less sensitive to the conditions of a standard PCR due to its primer size when compared to RAPD (random amplified polymorphic DNA) and hence are more specific and reproducible. Moreover, SCARs require no radioactive isotopes and detect only a single locus. Here, we describe the development of SCAR from RAPD amplicons.
Key wordsSCAR Polymorphisms DNA marker Genotyping
- 7.Trebaol G, Manceau C, Tirilly Y, et al (2001) Assessment of the genetic diversity among strains of Xanthomonas cynarae by randomly amplified polymorphic DNA analysis and development of specific characterized amplified regions for the rapid identification of X. cynarae. Appl Environ Microbiol 67:3379–3384PubMedCrossRefGoogle Scholar
- 9.Sambrook J, Russell DW(2001) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, New York.Google Scholar