Abstract
The technique of intracerebroventricular (i.c.v.) injection is a useful neuroethological tool for studying the central actions of drugs on behavior of conscious animals because many drugs do not cross the blood–brain barrier, which limits the extent of drug action to the CNS. In this chapter we propose a rapid, safe, and inexpensive method for i.c.v. injection in adult zebrafish which does not require implantation of a guide cannula.
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Acknowledgment
This research was supported by FAPESP Grant number 2008/57451.6. The authors would like to thank Dr. Keith C. Cheng and NCRR Funding by kind permission to use a figure from Zebrafish Virtual Atlas sponsored by NIH Grant R24RR017441.
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Barbosa, A. et al. (2012). Rapid Method for Acute Intracerebroventricular Injection in Adult Zebrafish. In: Kalueff, A., Stewart, A. (eds) Zebrafish Protocols for Neurobehavioral Research. Neuromethods, vol 66. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-597-8_25
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DOI: https://doi.org/10.1007/978-1-61779-597-8_25
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