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Assessment of Neurovascular Coupling

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Animal Models of Acute Neurological Injuries II

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Animal models are of great importance for studies on regional cerebral blood flow (rCBF) regulation in the normal and diseased brain. The complex interplay among neuronal, metabolic, and vascular signaling cascades in the regulation of brain blood flow requires its study in the intact living brain. This chapter provides a description of animal surgery, and explains how to implant cranial windows in order to investigate mechanisms of neurovascular coupling. Frequently used methods for the assessment of rCBF in response to neuronal depolarization are explained.

Cecilia Nicoletti and Nikolas Offenhauser contributed equally to this work.

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Acknowledgment

Supported by grants of the Deutsche Forschungsgemeinschaft (DFG DR 323/3-1), the Bundesministerium für Bildung und Forschung (Center for Stroke Research Berlin, 01 EO 0801), and the Kompetenznetz Schlaganfall to Dr. Dreier.

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Authors and Affiliations

  1. Center for Stroke Research, Berlin, Germany

    Cecilia Nicoletti, Nikolas Offenhauser, Devi Jorks, Sebastian Major & Jens P. Dreier

  2. Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany

    Cecilia Nicoletti, Nikolas Offenhauser, Devi Jorks & Jens P. Dreier

  3. Department of Neurology and Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany

    Sebastian Major

  4. Department of Neurology, Charité University Medicine Berlin, Berlin, Germany

    Jens P. Dreier

Authors
  1. Cecilia Nicoletti
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  2. Nikolas Offenhauser
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  3. Devi Jorks
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  4. Sebastian Major
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  5. Jens P. Dreier
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Corresponding author

Correspondence to Jens P. Dreier .

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Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Nicoletti, C., Offenhauser, N., Jorks, D., Major, S., Dreier, J.P. (2012). Assessment of Neurovascular Coupling. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-576-3_24

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  • DOI: https://doi.org/10.1007/978-1-61779-576-3_24

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