Multifluorescence 2D Gel Imaging and Image Analysis

  • Ingo Vormbrock
  • Sonja Hartwig
  • Stefan LehrEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 854)


Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.

Key words

2DE Two-dimensional gel electrophoresis Cyanine fluorescence dyes Gel imaging Image analysis 2DE Multifluorescence analysis Multifluorescence analysis Protein visualization Spot detection DIGE 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Clinical Biochemistry and PathobiochemistryGerman Diabetes CenterDüsseldorfGermany

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