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Multifluorescence 2D Gel Imaging and Image Analysis

  • Ingo Vormbrock
  • Sonja Hartwig
  • Stefan LehrEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 854)

Abstract

Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.

Key words

2DE Two-dimensional gel electrophoresis Cyanine fluorescence dyes Gel imaging Image analysis 2DE Multifluorescence analysis Multifluorescence analysis Protein visualization Spot detection DIGE 

References

  1. 1.
    GE Healthcare Handbook: Fluorescence imaging: Principles and Methods, GE Healthcare Life Science (2002) 63-0035–28.Google Scholar
  2. 2.
    Rozanas CR, Loyland SM (2008) Capabilities using 2-D DIGE in proteomics research: the new gold standard for 2-D gel electrophoresis. in: Liu BCS, Ehrlich JR, Eds. Tissue Proteomics: Approaches for Pathways, Biomarkers, and Drug Discovery. Humana Press, Totowa NJ. 441:1–18Google Scholar
  3. 3.
    Luhn S, Berth M, Hecker M, Bernhardt J (2003) Using standard positions and image fusion to create proteome maps from collections of two-dimensional gel electrophoresis images. Proteomics. 3(7):1117–27.PubMedCrossRefGoogle Scholar
  4. 4.
    Friedman DB, Hill S, Keller JW, Merchant NB, Levy SE, Coffey RJ, Caprioli RM (2004) Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics. 4(3):793–811.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Clinical Biochemistry and PathobiochemistryGerman Diabetes CenterDüsseldorfGermany

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