Multifluorescence 2D Gel Imaging and Image Analysis
Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.
Key words2DE Two-dimensional gel electrophoresis Cyanine fluorescence dyes Gel imaging Image analysis 2DE Multifluorescence analysis Multifluorescence analysis Protein visualization Spot detection DIGE
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