Method for Protein Subfractionation of Cardiovascular Tissues Before DIGE Analysis

  • Athanasios Didangelos
  • Xiaoke Yin
  • Manuel MayrEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 854)


Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071–2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.

Key words

Cardiovascular Heart Proteomics Vascular Vessel 



M.M. is a senior fellow of the British Heart Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Athanasios Didangelos
    • 1
  • Xiaoke Yin
    • 1
  • Manuel Mayr
    • 1
    Email author
  1. 1.Cardiovascular DivisionKing’s College LondonLondonUK

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