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Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery

  • Megan Penno
  • Matthias Ernst
  • Peter HoffmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 854)

Abstract

Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described.

Key words

Biomarkers Serum Depletion Multiple affinity removal system Tryptic digestion 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Adelaide Proteomics CentreUniversity of AdelaideAdelaideAustralia
  2. 2.Ludwig Centre for Cancer ResearchParkvilleAustralia

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