Skip to main content
SpringerLink
Log in

Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury

  • Protocol
  • First Online:
Book cover Pain Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 851))

Abstract

The spinal nerve ligation model of neuropathic pain in rats, as originally described by Kim and Chung (Pain 50:355–363, 1992), provides an excellent venue to study the antinociception and modulation effects of pulsed radiofrequency (PRF) current in pain processing. We describe the procedure of application of PRF current near the exposed L5 dorsal root ganglion (DRG) in rats with L5 spinal nerve ligation injury-induced behavioral hypersensitivity. This method employs the direct visualization of the L5 DRG, allowing for confirmation of the location of the PRF probe adjacent to the DRG.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. von Boxem K, van Eerd M, Brinkuize T, Patijn J, van Kleef M, van Zundert J. (2008) Radiofrequency and pulsed radiofrequency radiofrequency treatment of chronic pain syndromes: the available evidence. Pain Practice 8, 385–93.

    Google Scholar 

  2. Orlandini, G. (2004) Pulsed percutaneous radiofrequency treatment of the Gasserian ganglion for therapy of trigeminal neuralgia: technical notes, validity of the method and selection of the patients. Pain 108, 297–8.

    Article  PubMed  Google Scholar 

  3. Haider, N., Mekasha, D., Chiravuri, S., and Wasserman, R. (2007) Pulsed radiofrequency of the median nerve under ultrasound guidance. Pain Physician 10, 765–70.

    PubMed  Google Scholar 

  4. Navani, A., Mahajan, G., Kreis, P., and Fishman, S. M. (2006) A case of pulsed radiofrequency lesioning for occipital neuralgia. Pain Med 7, 453–6.

    Article  PubMed  Google Scholar 

  5. Cohen, S. P., Sireci, A., Wu, C. L., Larkin, T. M., Williams, K. A., and Hurley, R. W. (2006) Pulsed radiofrequency of the dorsal root ganglia is superior to pharmacotherapy or pulsed radiofrequency of the intercostal nerves in the treatment of chronic postsurgical thoracic pain. Pain Physician 9, 227–35.

    PubMed  Google Scholar 

  6. Rhame, E. E., Levey, K. A., and Gharibo, C. G. (2009) Successful treatment of refractory pudendal neuralgia with pulsed radiofrequency. Pain Physician 12, 633–8.

    PubMed  Google Scholar 

  7. Mitra, R., Zeighami, A., and Mackey, S. (2007) Pulsed radiofrequency for the treatment of chronic ilioinguinal neuropathy. Hernia 11, 369–71.

    Article  PubMed  Google Scholar 

  8. Rozen, D., and Ahn, J. (2006) Pulsed radiofrequency for the treatment of ilioinguinal neuralgia after inguinal herniorrhaphy. Mt Sinai J Med 73, 716–8.

    PubMed  Google Scholar 

  9. Shah, R. V., and Racz, G. B. (2003) Pulsed mode radiofrequency lesioning to treat chronic post-tonsillectomy pain (secondary glossopharyngeal neuralgia). Pain Pract 3, 232–7.

    Article  PubMed  Google Scholar 

  10. Tamimi, M. A., McCeney, M. H., and Krutsch, J. (2009) A case series of pulsed radiofrequency treatment of myofascial trigger points and scar neuromas. Pain Med 10, 1140–3.

    Article  PubMed  Google Scholar 

  11. Sluijter, M. E., Teixeira, A., Serra, V., Balogh, S., and Schianchi, P. (2008) Intra-articular application of pulsed radiofrequency for arthrogenic pain – report of six cases. Pain Pract 8, 57–61.

    Article  PubMed  Google Scholar 

  12. Rohof, O. J. (2002) Radiofrequency treatment of peripheral nerves. Pain Pract 2, 257–60.

    Article  PubMed  CAS  Google Scholar 

  13. Shah, R. V., and Racz, G. B. (2003) Pulsed mode radiofrequency lesioning of the suprascapular nerve for the treatment of chronic shoulder pain. Pain Physician 6, 503–6.

    PubMed  Google Scholar 

  14. Liliang, P. C., Lu, K., Liang, C. L., Tsai, Y. D., Hsieh, C. H., and Chen, H. J. (2009) Pulsed radiofrequency lesioning of the suprascapular nerve for chronic shoulder pain: a preliminary report. Pain Med 10, 70–5.

    Article  PubMed  Google Scholar 

  15. Kane, T. P., Rogers, P., Hazelgrove, J., Wimsey, S., and Harper, G. D. (2008) Pulsed radiofrequency applied to the suprascapular nerve in painful cuff tear arthropathy. J Shoulder Elbow Surg 17, 436–40.

    Article  PubMed  Google Scholar 

  16. Wu, H., and Groner, J. (2007) Pulsed radiofrequency treatment of articular branches of the obturator and femoral nerves for management of hip joint pain. Pain Pract 7, 341–4.

    Article  PubMed  Google Scholar 

  17. Teixeira, A., and Sluijter, M. E. (2006) Intradiscal high-voltage, long-duration pulsed radiofrequency for discogenic pain: a preliminary report. Pain Med 7, 424–8.

    Article  PubMed  Google Scholar 

  18. Vallejo, R., Benyamin, R. M., Kramer, J., Stanton, G., and Joseph, N. J. (2006) Pulsed radiofrequency denervation for the treatment of sacroiliac joint syndrome. Pain Med 7, 429–34.

    Article  PubMed  Google Scholar 

  19. Tekin, I., Mirzai, H., Ok, G., Erbuyun, K., and Vatansever, D. (2007) A comparison of conventional and pulsed radiofrequency denervation in the treatment of chronic facet joint pain. Clin J Pain 23, 524–9.

    Article  PubMed  Google Scholar 

  20. Kroll, H. R., Kim, D., Danic, M. J., Sankey, S. S., Gariwala, M., and Brown, M. (2008) A randomized, double-blind, prospective study comparing the efficacy of continuous versus pulsed radiofrequency in the treatment of lumbar facet syndrome. J Clin Anesth 20, 534–7.

    Article  PubMed  Google Scholar 

  21. Simopoulos, T. T., Kraemer, J., Nagda, J. V., Aner, M., and Bajwa, Z. H. (2008) Response to pulsed and continuous radiofrequency lesioning of the dorsal root ganglion and segmental nerves in patients with chronic lumbar radicular pain. Pain Physician 11, 137–44.

    PubMed  Google Scholar 

  22. Van Zundert, J., Patijn, J., Kessels, A., Lame, I., van Suijlekom, H., and van Kleef, M. (2007) Pulsed radiofrequency adjacent to the cervical dorsal root ganglion in chronic cervical radicular pain: a double blind sham controlled randomized clinical trial. Pain 127, 173–82.

    Article  PubMed  Google Scholar 

  23. Ozsoylar, O., Akcali, D., Cizmeci, P., Babacan, A., Cahana, A., and Bolay, H. (2008) Percutaneous pulsed radiofrequency reduces mechanical allodynia in a neuropathic pain model. Anesth Analg 107, 1406–11.

    Article  PubMed  Google Scholar 

  24. Aksu, R., Ugur, F., Bicer, C., Menku, A., Guler, G., Madenoglu, H., Canpolat, D. G., and Boyaci, A. (2010) The efficiency of pulsed radiofrequency application on L5 and L6 dorsal roots in rabbits developing neuropathic pain. Reg Anesth Pain Med 35, 11–5.

    Article  PubMed  Google Scholar 

  25. Hagiwara, S., Iwasaka, H., Takeshima, N., and Noguchi, T. (2009) Mechanisms of analgesic action of pulsed radiofrequency on adjuvant-induced pain in the rat: roles of descending adrenergic and serotonergic systems. Eur J Pain 13, 249–52.

    Article  PubMed  CAS  Google Scholar 

  26. Perret, D. M., Kim, D.-S., Li, K.-W., Sinavsky, K., Newcomb, R. L., Miller, J. M., and Luo, Z. D. (2011) Application of Pulsed Radiofrequency Currents to Rat Dorsal Root Ganglia Modulates Nerve Injury-Induced Tactile Allodynia. Anesthesia & Analgesia 113, 610–16.

    Google Scholar 

  27. Sluijter, M. E., Cosman, E. R., Rittman, W. B., and van Kleef, M. (1998) The effects of pulsed radiofrequency fields applied to the dorsal root ganglion. A preliminary report. Pain Clin 11, 109–17.

    Google Scholar 

  28. Hildebrandt, J. (2001) Relevance of nerve blocks in treating and diagnosing low back pain – is the quality decisive?. Schmerz 15, 474–83.

    Article  PubMed  CAS  Google Scholar 

  29. Erdine, S., Bilir, A., Cosman, E. R., and Cosman, E. R., Jr. (2009) Ultrastructural changes in axons following exposure to pulsed radiofrequency fields. Pain Pract 9, 407–17.

    Article  PubMed  Google Scholar 

  30. Higuchi, Y., Nashold, B. S., Jr., Sluijter, M., Cosman, E., and Pearlstein, R. D. (2002) Exposure of the dorsal root ganglion in rats to pulsed radiofrequency currents activates dorsal horn lamina I and II neurons. Neurosurgery 50, 850–5; discussion 6.

    Google Scholar 

  31. Van Zundert, J., de Louw, A. J., Joosten, E. A., Kessels, A. G., Honig, W., Dederen, P. J., Veening, J. G., Vles, J. S., and van Kleef, M. (2005) Pulsed and continuous radiofrequency current adjacent to the cervical dorsal root ganglion of the rat induces late cellular activity in the dorsal horn. Anesthesiology 102, 125–31.

    Article  PubMed  Google Scholar 

  32. Podhajsky, R. J., Sekiguchi, Y., Kikuchi, S., and Myers, R. R. (2005) The histologic effects of pulsed and continuous radiofrequency lesions at 42 degrees C to rat dorsal root ganglion and sciatic nerve. Spine (Phila Pa 1976) 30, 1008–13.

    Google Scholar 

  33. Hamann, W., Abou-Sherif, S., Thompson, S., and Hall, S. (2006) Pulsed radiofrequency applied to dorsal root ganglia causes a selective increase in ATF3 in small neurons. Eur J Pain 10, 171–6.

    Article  PubMed  CAS  Google Scholar 

  34. Chung, J. M., Kim, H. K., and Chung, K. (2004) Segmental spinal nerve ligation model of neuropathic pain. Methods Mol Med 99, 35–45.

    PubMed  Google Scholar 

  35. Higuera, E. S., and Luo, Z. D. (2004) A rat pain model of vincristine-induced neuropathy. Methods Mol Med 99, 91–8.

    PubMed  CAS  Google Scholar 

  36. Allen, J. W., and Yaksh, T. L. (2004) Assessment of acute thermal nociception in laboratory animals, in Pain Research: Methods and Protocols (Luo, Z. D., Ed.), pp 11–23, Humana Press Inc., Totowa.

    Google Scholar 

  37. Kim, S. H., and Chung, J. M. (1992) An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain 50, 355–63.

    Article  PubMed  CAS  Google Scholar 

  38. Luo, Z. D., Chaplan, S. R., Higuera, E. S., Sorkin, L. S., Stauderman, K. A., Williams, M. E., and Yaksh, T. L. (2001) Upregulation of dorsal root ganglion (alpha)2(delta) calcium channel subunit and its correlation with allodynia in spinal nerve-injured rats. J Neurosci 21, 1868–75.

    PubMed  CAS  Google Scholar 

  39. Wang, R., Guo, W., Ossipov, M. H., Vanderah, T. W., Porreca, F., and Lai, J. (2003) Glial cell line-derived neurotrophic factor normalizes neurochemical changes in injured dorsal root ganglion neurons and prevents the expression of experimental neuropathic pain. Neuroscience 121, 815–24.

    Article  PubMed  CAS  Google Scholar 

  40. Takeda, K., Sawamura, S., Tamai, H., Sekiyama, H., and Hanaoka, K. (2005) Role for cyclooxygenase 2 in the development and maintenance of neuropathic pain and spinal glial activation. Anesthesiology 103, 837–44.

    Article  PubMed  CAS  Google Scholar 

  41. Chaplan, S. R., Bach, F. W., Pogrel, J. W., Chung, J. M., and Yaksh, T. L. (1994) Quantitative assessment of tactile allodynia in the rat paw. Journal of Neuroscience Methods 53, 55–63.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by pilot grants from the Committee on Research and Graduate Academic Programs and the Department of Anesthesiology & Perioperative Care, University of California Irvine Medical Center (D. Perret). The authors would like to thank Mr. Joshua Lee for his technical assistance in preparing the figure.

Author information

Authors and Affiliations

  1. Department of Anesthesiology & Perioperative Care, School of Medicine, University of California Irvine, Irvine, CA, USA

    Danielle Perret, Kang-Wu Li & Z. David Luo

  2. Department of Physical Medicine & Rehabilitation, School of Medicine, University of California Irvine, Irvine, CA, USA

    Danielle Perret

  3. Department of Anesthesia and Pain Medicine, School of Medicine, Kosin University, Busan, Republic of Korea

    Doo-Sik Kim

  4. Department of Pharmacology, School of Medicine, University of California Irvine, Irvine, CA, USA

    Z. David Luo

Authors
  1. Danielle Perret
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Doo-Sik Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kang-Wu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z. David Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z. David Luo .

Editor information

Editors and Affiliations

  1. Irvine Medical Center, Dept. Anesthesiology & Pharmacology, University of California, The City Drive S. 101, Orange, 92868, California, USA

    Z. David Luo

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Perret, D., Kim, DS., Li, KW., Luo, Z.D. (2012). Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury. In: Luo, Z. (eds) Pain Research. Methods in Molecular Biology, vol 851. Humana Press. https://doi.org/10.1007/978-1-61779-561-9_21

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-561-9_21

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-560-2

  • Online ISBN: 978-1-61779-561-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation