Abstract
Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
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Acknowledgements
Joanne Gibson’s PhD studentship was sponsored by SUMMIT plc and BBSRC. We also thank Tenovus Scotland, University of Aberdeen (SW and JG) and the Wellcome Trust (Grant 08088) for their support in our research in this area.
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Gibson, J., Gow, N.A.R., Wong, S.Y.C. (2012). Murine Bone Marrow-Derived Dendritic Cells and T-Cell Activation by Candida albicans . In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_17
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DOI: https://doi.org/10.1007/978-1-61779-539-8_17
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