Abstract
Epicardium is the outer cell layer of the heart. Its integrity and function are essential for normal heart development. To study the role of epicardium in both fetal and adult hearts, it is desirable to isolate and culture pure populations of these cells. Here we describe methods with Cre-loxP technology to lineage tag epicardial cells (EpiCs) and epicardium-derived cells (EPDCs), dissociate and isolate them by flow-activated cytometry sorting (FACS), and characterize them by quantitative PCR and immunostaining. This platform allows further characterization and manipulation of EpiCs and EPDCs for expression studies and functional assays.
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Acknowledgments
The authors would like to thank Elizabeth Boush for aiding FACS isolation. This work was supported by funding from NIH RO1 HL094683 (WTP), an American Heart Association Postdoctoral Fellowship (BZ), and charitable support from James Smith and Gail Federici-Smith (WTP), and the Simeon Burt Wolbach Research Fund (BZ).
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Zhou, B., Pu, W.T. (2012). Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_15
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DOI: https://doi.org/10.1007/978-1-61779-523-7_15
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